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cd163 microbead kit  (Miltenyi Biotec)


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    Miltenyi Biotec cd163 microbead kit
    Senescence-like TAMs in clinical specimens. (A) IF analysis of p16 + <t>CD163</t> + TAMs in treatment-naïve TNBC (direct surgery, n = 20) versus post-neoadjuvant chemotherapy specimens ( n = 17). (B) IF analysis of p16 + CD163 + TAMs in post-chemotherapy samples from patients with progressive disease (PD, n = 5) versus complete/partial remission (CR/PR, n = 12). (C) The senescence-like TAM gene signature scores across different sample groups: complete response, partial response, stable disease, and progressive disease. Data was from the BRCA-TCGA cohort and analyzed by GEPIA3 ( https://gepia3.bioinfoliu.com/ ). (D) The prognostic value of senescence-like TAM gene signature in cases with Carboplatin treatment. For statistical analyses, an unpaired two-tailed t-test (A and B) or log-rank test (E) was used. ** P < 0.01 and **** P < 0.0001
    Cd163 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd163+microbead+kit/CD163+MicroBead+Kit%2C+human/pmc13009420-48-24-27
    Average 94 stars, based on 14 article reviews
    cd163 microbead kit - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancer"

    Article Title: Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancer

    Journal: Cellular Oncology

    doi: 10.1007/s13402-026-01197-3

    Senescence-like TAMs in clinical specimens. (A) IF analysis of p16 + CD163 + TAMs in treatment-naïve TNBC (direct surgery, n = 20) versus post-neoadjuvant chemotherapy specimens ( n = 17). (B) IF analysis of p16 + CD163 + TAMs in post-chemotherapy samples from patients with progressive disease (PD, n = 5) versus complete/partial remission (CR/PR, n = 12). (C) The senescence-like TAM gene signature scores across different sample groups: complete response, partial response, stable disease, and progressive disease. Data was from the BRCA-TCGA cohort and analyzed by GEPIA3 ( https://gepia3.bioinfoliu.com/ ). (D) The prognostic value of senescence-like TAM gene signature in cases with Carboplatin treatment. For statistical analyses, an unpaired two-tailed t-test (A and B) or log-rank test (E) was used. ** P < 0.01 and **** P < 0.0001
    Figure Legend Snippet: Senescence-like TAMs in clinical specimens. (A) IF analysis of p16 + CD163 + TAMs in treatment-naïve TNBC (direct surgery, n = 20) versus post-neoadjuvant chemotherapy specimens ( n = 17). (B) IF analysis of p16 + CD163 + TAMs in post-chemotherapy samples from patients with progressive disease (PD, n = 5) versus complete/partial remission (CR/PR, n = 12). (C) The senescence-like TAM gene signature scores across different sample groups: complete response, partial response, stable disease, and progressive disease. Data was from the BRCA-TCGA cohort and analyzed by GEPIA3 ( https://gepia3.bioinfoliu.com/ ). (D) The prognostic value of senescence-like TAM gene signature in cases with Carboplatin treatment. For statistical analyses, an unpaired two-tailed t-test (A and B) or log-rank test (E) was used. ** P < 0.01 and **** P < 0.0001

    Techniques Used: Two Tailed Test

    Related Articles

    Purification:

    Article Title: TLR8 agonist selgantolimod regulates Kupffer cell differentiation status and impairs HBV entry into hepatocytes via an IL-6-dependent mechanism
    Article Snippet: PHH were cultured on a collagen layer and maintained in Williams E medium (Gibco, Billings, MT, USA) supplemented with 5% FCII serum (Cytiva, Marlborough, MA, USA), 50 U/mL of penicillin/streptomycin, 5 μg/mL of bovine insulin, 2% DMSO (Sigma-Aldrich, St. Louis, MO, USA), 1x Glutamax (Gibco) and 5×10 −5 M of hydrocortisone (SERB, Brussels, BE). .. KCs were purified from the non-parenchymal cell mixture by a two-phase iodixanol gradient (Optiprep, BioVision, Waltham, MA, USA), followed by positive selection with the CD163 MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, DE). .. KCs were seeded at 3×10 5 cells/well into 24-well plates and cultured in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCII serum and 50 U/mL of penicillin/streptomycin.

    Article Title: TLR8 agonist selgantolimod regulates Kupffer cell differentiation status and impairs HBV entry into hepatocytes via an IL-6-dependent mechanism.
    Article Snippet: METHODS Primary cell isolation and culture After a two- step collagenase perfusion, the liver extract was filtered and centrifuged, as previously described.18 PHH were cultured on a collagen layer and maintained in Williams E medium (Gibco, Billings, MT, USA) supplemented with 5% FCII serum (Cytiva, Marlborough, MA, USA), 50 U/mL of penicillin/streptomycin, 5 μg/mL of bovine insulin, 2% DMSO (Sigma- Aldrich, St. Louis, MO, USA), 1x Glutamax (Gibco) and 5×10−5 M of hydrocortisone (SERB, Brussels, BE). .. KCs were purified from the non- parenchymal cell mixture by a two- phase iodixanol gradient (Optiprep, BioVision, Waltham, MA, USA), followed by positive selection with the CD163 MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, DE). .. KCs were seeded at 3×105 cells/well into 24- well plates and cultured in RPMI- 1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCII serum and 50 U/mL of penicillin/streptomycin.

    Selection:

    Article Title: TLR8 agonist selgantolimod regulates Kupffer cell differentiation status and impairs HBV entry into hepatocytes via an IL-6-dependent mechanism
    Article Snippet: PHH were cultured on a collagen layer and maintained in Williams E medium (Gibco, Billings, MT, USA) supplemented with 5% FCII serum (Cytiva, Marlborough, MA, USA), 50 U/mL of penicillin/streptomycin, 5 μg/mL of bovine insulin, 2% DMSO (Sigma-Aldrich, St. Louis, MO, USA), 1x Glutamax (Gibco) and 5×10 −5 M of hydrocortisone (SERB, Brussels, BE). .. KCs were purified from the non-parenchymal cell mixture by a two-phase iodixanol gradient (Optiprep, BioVision, Waltham, MA, USA), followed by positive selection with the CD163 MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, DE). .. KCs were seeded at 3×10 5 cells/well into 24-well plates and cultured in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCII serum and 50 U/mL of penicillin/streptomycin.

    Article Title: TLR8 agonist selgantolimod regulates Kupffer cell differentiation status and impairs HBV entry into hepatocytes via an IL-6-dependent mechanism.
    Article Snippet: METHODS Primary cell isolation and culture After a two- step collagenase perfusion, the liver extract was filtered and centrifuged, as previously described.18 PHH were cultured on a collagen layer and maintained in Williams E medium (Gibco, Billings, MT, USA) supplemented with 5% FCII serum (Cytiva, Marlborough, MA, USA), 50 U/mL of penicillin/streptomycin, 5 μg/mL of bovine insulin, 2% DMSO (Sigma- Aldrich, St. Louis, MO, USA), 1x Glutamax (Gibco) and 5×10−5 M of hydrocortisone (SERB, Brussels, BE). .. KCs were purified from the non- parenchymal cell mixture by a two- phase iodixanol gradient (Optiprep, BioVision, Waltham, MA, USA), followed by positive selection with the CD163 MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, DE). .. KCs were seeded at 3×105 cells/well into 24- well plates and cultured in RPMI- 1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCII serum and 50 U/mL of penicillin/streptomycin.

    other:

    Article Title: Protocol for isolating CD163 + Kupffer cells from human liver resections
    Article Snippet: CD163 MicroBead kit (containing CD163-biotin and anti-biotin microBeads) , Miltenyi Biotec , 130-124-420.

    FACS:

    Article Title: Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancer
    Article Snippet: .. Endothelial cells were enriched by magnetic-activated cell sorting using the CD31 MicroBead Kit (Miltenyi Biotec Cat# 130-091-935, RRID: AB_3699142), TAMs were enriched with the CD163 MicroBead Kit (Miltenyi Biotec, Cat# 130-124-420), and CAFs were enriched using Anti-Fibroblast MicroBeads (Miltenyi Biotec, Cat# 130-050-601), in accordance with the manufacturers’ protocols. ..

    Article Title: Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancer.
    Article Snippet: .. Endothelial cells were enriched by magnetic-activated cell sorting using the CD31 MicroBead Kit (Miltenyi Biotec Cat# 130-091-935, RRID: AB_3699142), 1 3 61 Page 2 of 15 Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast... TAMs were enriched with the CD163 MicroBead Kit (Miltenyi Biotec, Cat# 130-124-420), and CAFs were enriched using Anti-Fibroblast MicroBeads (Miltenyi Biotec, Cat# 130-050-601), in accordance with the manufacturers’ protocols. ..



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    Senescence-like TAMs in clinical specimens. (A) IF analysis of p16 + <t>CD163</t> + TAMs in treatment-naïve TNBC (direct surgery, n = 20) versus post-neoadjuvant chemotherapy specimens ( n = 17). (B) IF analysis of p16 + CD163 + TAMs in post-chemotherapy samples from patients with progressive disease (PD, n = 5) versus complete/partial remission (CR/PR, n = 12). (C) The senescence-like TAM gene signature scores across different sample groups: complete response, partial response, stable disease, and progressive disease. Data was from the BRCA-TCGA cohort and analyzed by GEPIA3 ( https://gepia3.bioinfoliu.com/ ). (D) The prognostic value of senescence-like TAM gene signature in cases with Carboplatin treatment. For statistical analyses, an unpaired two-tailed t-test (A and B) or log-rank test (E) was used. ** P < 0.01 and **** P < 0.0001
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    Image Search Results


    Senescence-like TAMs in clinical specimens. (A) IF analysis of p16 + CD163 + TAMs in treatment-naïve TNBC (direct surgery, n = 20) versus post-neoadjuvant chemotherapy specimens ( n = 17). (B) IF analysis of p16 + CD163 + TAMs in post-chemotherapy samples from patients with progressive disease (PD, n = 5) versus complete/partial remission (CR/PR, n = 12). (C) The senescence-like TAM gene signature scores across different sample groups: complete response, partial response, stable disease, and progressive disease. Data was from the BRCA-TCGA cohort and analyzed by GEPIA3 ( https://gepia3.bioinfoliu.com/ ). (D) The prognostic value of senescence-like TAM gene signature in cases with Carboplatin treatment. For statistical analyses, an unpaired two-tailed t-test (A and B) or log-rank test (E) was used. ** P < 0.01 and **** P < 0.0001

    Journal: Cellular Oncology

    Article Title: Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancer

    doi: 10.1007/s13402-026-01197-3

    Figure Lengend Snippet: Senescence-like TAMs in clinical specimens. (A) IF analysis of p16 + CD163 + TAMs in treatment-naïve TNBC (direct surgery, n = 20) versus post-neoadjuvant chemotherapy specimens ( n = 17). (B) IF analysis of p16 + CD163 + TAMs in post-chemotherapy samples from patients with progressive disease (PD, n = 5) versus complete/partial remission (CR/PR, n = 12). (C) The senescence-like TAM gene signature scores across different sample groups: complete response, partial response, stable disease, and progressive disease. Data was from the BRCA-TCGA cohort and analyzed by GEPIA3 ( https://gepia3.bioinfoliu.com/ ). (D) The prognostic value of senescence-like TAM gene signature in cases with Carboplatin treatment. For statistical analyses, an unpaired two-tailed t-test (A and B) or log-rank test (E) was used. ** P < 0.01 and **** P < 0.0001

    Article Snippet: Endothelial cells were enriched by magnetic-activated cell sorting using the CD31 MicroBead Kit (Miltenyi Biotec Cat# 130-091-935, RRID: AB_3699142), TAMs were enriched with the CD163 MicroBead Kit (Miltenyi Biotec, Cat# 130-124-420), and CAFs were enriched using Anti-Fibroblast MicroBeads (Miltenyi Biotec, Cat# 130-050-601), in accordance with the manufacturers’ protocols.

    Techniques: Two Tailed Test

    Titin-expressing macrophages are present in the choroid plexus. (A) Heatmap of the top eight differentially expressed genes in each of the three ChP macrophage sub-clusters. Gene expression levels are represented on a normalized gradient scale. (B) DAB immunohistochemical stain of a 5 µm FFPE section of control ChP tissue using a CD163 antibody. CD163-positive macrophages are marked by brown staining, while cell nuclei are counterstained blue with hematoxylin, indicating their distribution within the vascularized ChP tissue. Scale bar = 40 µm. (C) Violin plots illustrating TTN expression levels in ChP samples from Alzheimer’s disease and control conditions. Each plot shows the distribution, density, and variability of TTN transcript counts in ChP macrophages within each condition. (D) Schematic representation of the experimental workflow for evaluation of TTN RNA enrichment in macrophages, involving tissue dissociation, CD163 positive cell enrichment, RNA isolation, cDNA synthesis, PCR amplification using isoform-specific TTN primers, and ΔΔCq calculation with GAPDH used for normalization. (E) Fold changes, computed from ΔΔCq, in TTN isoform expression relative to GAPDH in CD163-bead enriched macrophages relative to whole tissue. The x-axis represents different primer sets (1, 3, 7, and 12), and the y-axis shows fold changes (log scale). Each point represents a single donor sample/primer set pair. (F) Schematic representation of the TTN gene with highlighted primer binding sites, with genomic coordinates on chromosome 2, indicating selected splicing sites and isoforms of TTN. (G) Immunofluorescence images of ChP with anti-CD68 (red) and anti-titin (green) primary antibodies, co-stained with DAPI (blue), showing cytoplasmic titin protein in ChP macrophage cytoplasm. Merged images are in right column. Scale bars = 20 µm.

    Journal: bioRxiv

    Article Title: Mechanobiological Specialization of Choroid Plexus Macrophages Defined by Titin Expression

    doi: 10.64898/2026.01.20.700716

    Figure Lengend Snippet: Titin-expressing macrophages are present in the choroid plexus. (A) Heatmap of the top eight differentially expressed genes in each of the three ChP macrophage sub-clusters. Gene expression levels are represented on a normalized gradient scale. (B) DAB immunohistochemical stain of a 5 µm FFPE section of control ChP tissue using a CD163 antibody. CD163-positive macrophages are marked by brown staining, while cell nuclei are counterstained blue with hematoxylin, indicating their distribution within the vascularized ChP tissue. Scale bar = 40 µm. (C) Violin plots illustrating TTN expression levels in ChP samples from Alzheimer’s disease and control conditions. Each plot shows the distribution, density, and variability of TTN transcript counts in ChP macrophages within each condition. (D) Schematic representation of the experimental workflow for evaluation of TTN RNA enrichment in macrophages, involving tissue dissociation, CD163 positive cell enrichment, RNA isolation, cDNA synthesis, PCR amplification using isoform-specific TTN primers, and ΔΔCq calculation with GAPDH used for normalization. (E) Fold changes, computed from ΔΔCq, in TTN isoform expression relative to GAPDH in CD163-bead enriched macrophages relative to whole tissue. The x-axis represents different primer sets (1, 3, 7, and 12), and the y-axis shows fold changes (log scale). Each point represents a single donor sample/primer set pair. (F) Schematic representation of the TTN gene with highlighted primer binding sites, with genomic coordinates on chromosome 2, indicating selected splicing sites and isoforms of TTN. (G) Immunofluorescence images of ChP with anti-CD68 (red) and anti-titin (green) primary antibodies, co-stained with DAPI (blue), showing cytoplasmic titin protein in ChP macrophage cytoplasm. Merged images are in right column. Scale bars = 20 µm.

    Article Snippet: RNA was extracted from CD163 microbead-separated macrophages (Miltenyi Biotec, Cat. #130-124-420) using the RNeasy Mini Kit (Qiagen), following the manufacturer’s protocol.

    Techniques: Expressing, Gene Expression, Immunohistochemical staining, Staining, Control, Isolation, cDNA Synthesis, Amplification, Binding Assay, Immunofluorescence